|
KMID : 0895820220320010001
|
|
Journal of Oriental Rehabilitation Medicine
2022 Volume.32 No. 1 p.1 ~ p.19
|
|
|
Effects of Oral Administered Hot Water Extracts of Korean Black Ginseng on Wound Healing in Mice
|
|
Kim Tae-Ryeong
Kim Young-Jun
Woo Chang-Hoon
|
|
|
Abstract
|
|
|
Objectives This study aims to evaluate the wound healing effects of oral administered hot water extracts of Korean black ginseng (KBG).
Methods 40 C57BL/6 mice were divided into five groups; normal, control, vitamin E 200 mg/kg, KBG 100 mg/kg, KBG 200 mg/kg, each n=8. Skin wounds were made in the back of all mice except normal group using biopsy punches. Wounds were observed on days 7 and 14 after injury. The anti-oxidant and inflammatory protein levels were evaluated using western blotting. Skin tissue was analyzed by hematoxylin & eosin and Masson’s trichrome staining method.
Results KBG significantly accelerated reducing wound area. KBG significantly decreased myeloperoxidase activity. KBG significantly decreased oxidative stress factors such as NADPH oxidase-4 and p22phox and increased antioxidant enzymes including nuclear factor erythroid 2-related factor2, kelch-like ECH-associated protein- 1, heme oxygenase-1, superoxide dismutase, catalase and glutathione peroxidase- 1/2. Moreover, KBG significantly decreased inflammation factors including nuclear factor-κB, phosphorylated inhibitor of κBα, cyclooxygenase-2, inducible nitric oxide synthase, tumor necrosis factor-α and interleukin (IL)-6 and increased anti-inflammation cytokine such as IL-4 and IL-10. In addition, KBG significantly increased tight junction proteins including claudin-1, claudin-3, claudin-4. In histopathologic, KBG made the epithelium thin and uniform, and accelerated the remodeling of collagen.
Conclusions The results suggest that KBG has healing effects on skin wound in mice by anti-inflammatory and antioxidant activity.
|
|
|
KEYWORD
|
|
|
Panax, Wound healing, Wounds and injuries, Anti-inflammatory agents, Antioxidants
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|